Patents

Authors: Shishir Chundawat, Bhargava Nemmaru, Antonio DeChellis, Deanne Sammond

Patent Link: Link

Abstract: Compositions and methods for supercharging 5 endocellulases Cel5A and its native family 2a CBM from the thermophilic microbe Thermobifida fusca for efficient degradation of biomass, particularly recalcitrant substrates such as, cellulose and corn stover, are described.

Authors: Shishir Chundawat, Aron Gyorgypal

Patent Link: Link

Abstract: At least some embodiments disclosed here may be directed to design and implementation of a novel PAT tool for real-time (or near real-time) online N-glycosylation analysis through the use of sequential injection analysis (SIA) coupled with liquid chromatography; rapid and robust N-glycosylation monitoring during N-glycosylated protein (e.g., monoclonal antibody) bioprocessing enabled by instant, sensitive labeling chemistry; and systems thereof.

Authors: Shishir Chundawat, Chandra Kanth Bandi

Patent Link: Link

Abstract: The present invention relates to engineered carbohydrate-active enzyme constructs that are useful for glycan polymers synthesis. The construct comprises a CD domain from a GH, wherein the domain is conjugated to CBM3a via a peptidic linker. The invention also relates to a method of improving glycan polymer synthesis by using engineered carbohydrate active enzymes comprising a CD domain and CBM3a.

Authors: Chandra Kanth Bandi, Shishir Chundawat, Kyle S.

Patent Link: Link

Abstract: The present disclosure provides, in one aspect, an azide-inducible system for controlled gene expression in E. coli. In another aspect, the present disclosure provides a high throughput screening method for the identification of new glycosynthases (e.g., mutant glycosyl hydrolases) with enhanced activity and unique substrate specificity.

Authors: Ayushi Agrawal, Chandra Kanth Bandi, Shishir Chundawat

Patent Link: Link

Abstract: The present disclosure relates, in one aspect, to the discovery of a high throughput screening (HTS) method to rapidly screen for GH/GS variants that are generated using directed evolution techniques and that can significantly enhance glycosynthase catalytic activity or product specificity.

Authors: Venkatesh Balan, Bruce E Dale, Shishir Chundawat, Leonardo Sousa

Patent Link: Link

Abstract: A method for pretreating biomass is provided, which includes, in a reactor, allowing gaseous ammonia to condense on the biomass and react with water present in the biomass to produce pretreated biomass, wherein reactivity of polysaccharides in the biomass is increased during subsequent biological conversion as compared to the reactivity of polysaccharides in biomass which has not been pretreated. A method for pretreating biomass with a liquid ammonia and recovering the liquid ammonia is also provided. Related systems which include a biochemical or biofuel production facility are also disclosed.

Authors: Venkatesh Balan, Shishir Chundawat, Leonardo Sousa, Bruce Dale

Patent Link: Link

Abstract: A process for producing sugars from lignocellulosic materials such as corn stover by the addition of corn stillage as a carbon source is disclosed. The sugars are formed by treating the combination of the corn stillage and the lignocellulosic materials with hydrolytic enzymes. The sugars can be fermented to ethanol, and the process improves ethanol production economies with an increased sugar yield from the lignocellulosic materials.

Authors: Ming Woei Lau, Bruce E. Dale, Venkatesh BalanShishir Chundawat

Patent Link: Link

 

Authors: Shishir Chundawat, Leonardo Da Costa Sousa, Albert M. Cheh, Venkatesh Balan, Bruce Dale

Patent Link: Link

Abstract: Methods for producing extracted and digested products from pretreated lignocellulosic biomass are provided. The methods include converting native cellulose Iβ to cellulose IIII by pretreating the lignocellulosic biomass with liquid ammonia under certain conditions, and performing extracting or digesting steps on the pretreated/converted lignocellulosic biomass.

Authors: Bruce E. Dale, Farzaneh Teymouri, Shishir Chundawat, Venkatesh Balan

Patent Link: Link

Abstract: The present invention describes a process for at least a 90% conversion of a plant biomass preferably by a reduction of the units of cellulase needed and by using a xylanase which acts synergistically with the cellulase to improve the yield of xylose and glucose as sugars. The process enables greater conversion of a lignocellulosic plant biomass to glucose and xylose for use as animal feeds and as fermentation as medium for producing ethanol.

Authors: Bruce Dale, Venkatesh Balan, Shishir Chundawat, Bryan Bals

Patent Link: Link

Abstract: A process for hydrolyzing whole grain or whole plant biomass after an Ammonia Fiber Explosion (AFEX) process step is described. The process preferably uses a biomass that is hydrolyzed using a different combination of enzymes (amylase, cellulase and hemicellulase) to sugars for fermentation to produce ethanol. Harvesting the whole plant inclusive of grains and stalk for ethanol bio-processing is an economical route for future biorefineries. In addition to sugars, various value-added products like proteins and oil can be co-generated.

Authors: Bruce Dale, Venkatesh Balan, Shishir Chundawat

Patent Link: Link

Abstract: A process for the conversion of monocot lignocellulosic grass waste from mushroom growth into byproducts is described. In particular, the present invention releases glucans from the waste which can be easily hydrolyzed, after a less severe thermochemical process (i.e. AFEX), and into sugars for producing ethanol or other by-products by fermentation.

Authors: Masud Huda, Venkatesh Balan, Lawrence Drzal, Bruce Dale, Shishir Chundawat, Manjusri Misra

Patent Link: Link

Abstract: A composite composition which comprises a synthetic polymer, and corncob granules which have been modified, such as with a chemical reacted with the hydroxyl groups on the granules is described. The corncob granules are modified so as to be compatible with the polymer.

Authors: Venkatesh Balan, Albert Cheh, Shishir Chundawat, Bruce E. Dale, Leonardo Sousa

Patent Link: Link

Abstract: A method is provided comprising converting at least a portion of native cellulose Iβ to cellulose IIII by treating plant biomass with liquid ammonia and/or one or more organic solvents to produce a treated plant biomass containing lignin and a lignin fraction, and extracting lignin and/or other plant cell wall components from the lignin fraction to produce a lignin extract capable of being fractionated. In one embodiment, the lignin extract is fractionated into separate components which are useful in a variety of applications.